normal prostate stromal immortalized cell line  (ATCC)

Journal: OncoTargets and therapy

Article Title: Knockdown of lncRNA TUG1 Enhances Radiosensitivity of Prostate Cancer via the TUG1/miR-139-5p/SMC1A Axis

doi: 10.2147/OTT.S236860

Figure Lengend Snippet: Expression of TUG1 and its effect on prognosis and radiosensitivity in PCa. ( A ) The expression of TUG1 was measured by qRT-PCR analysis in PCa tissues (n = 50) or the adjacent non-cancer tissues. ( B ) The expression of TUG1 was measured by qRT-PCR analysis in human normal prostatic stromal immortalized cell line (WPMY-1) and PCa cell lines (LNCaP, 22RV1, PC-3 and DU145). ( C ) Survival was analyzed and compared between patients with high and low levels of TUG1 using Kaplan–Meier analysis. ( D and E ) TUG1 expression was detected in PC-3 and DU145 cells every 3 hrs after 0 or 4 Gy of radiation treatment. * P < 0.05.

Article Snippet: Human normal prostatic stromal immortalized cell line (WPMY-1 (Dulbecco’s Modified Eagle’s Medium)) and PCa cells (LNCaP (RPMI 1640), 22RV1 (RPMI 1640), PC-3 (F-12K) and DU145 (Eagle’s Minimum Essential Medium)) were purchased from the American Type Culture Collection (ATCC, Manassas, VA, USA).

Techniques: Expressing, Quantitative RT-PCR

Journal: OncoTargets and therapy

Article Title: Knockdown of lncRNA TUG1 Enhances Radiosensitivity of Prostate Cancer via the TUG1/miR-139-5p/SMC1A Axis

doi: 10.2147/OTT.S236860

Figure Lengend Snippet: SMC1A was a target gene of miR-139-5p and the expression of SMC1A was upregulated in PCa cells. ( A ) The presumed binding sites of miR-139-5p with SMC1A were predicted. ( B and C ) The luciferase activity in PC-3 and DU145 cells simultaneously transfected with miR-139-5p mimics or mimics-NC and TUG1-WT or TUG1-MUT. ( D ) The expression of SMC1A was upregulated in PCa samples (n=50). ( E ) The relationship between SMC1A and miR-139-5p was analyzed by Kaplan–Meier analysis ( r = −0.5646, P < 0.001). ( F and G ) The expression levels of SMC1A mRNA and protein were overexpressed in PC-3 and DU145 cells comparing to human normal prostatic stromal immortalized cell line (WPMY-1). ( H and I ) The mRNA and protein expression of SMC1A in PC-3 and DU145 cells transfected with miR-139-5p mimics or mimics-NC were detected by qRT-PCR and Weston blot, respectively. * P < 0.05.

Article Snippet: Human normal prostatic stromal immortalized cell line (WPMY-1 (Dulbecco’s Modified Eagle’s Medium)) and PCa cells (LNCaP (RPMI 1640), 22RV1 (RPMI 1640), PC-3 (F-12K) and DU145 (Eagle’s Minimum Essential Medium)) were purchased from the American Type Culture Collection (ATCC, Manassas, VA, USA).

Techniques: Expressing, Binding Assay, Luciferase, Activity Assay, Transfection, Quantitative RT-PCR